使用機械分散(sàn)機(jī)(Dispermat)混(hùn)合(hé)疏水(shuǐ)蛋白,SDS和纖(xiān)維。分(fēn)散機速度可(kě)調(tiáo),但通(tōng)常(cháng)在(zài)實(shí)驗中使(shǐ)用(yòng)約3800rpm。研究泡沫體積隨時(shí)間的變化。通(tōng)過此(cǐ)設(shè)置,發泡在(zài)1分(fēn)鐘(zhōng)內達到最(zuì)大水平。通(tōng)過攝影(yǐng)監測泡沫(mò)體(tǐ)積(jī)的變化(huà),其(qí)中(zhōng)每10秒拍攝(shè)照(zhào)片。從(cóng)照片(piàn)圖像中(zhōng),確定(dìng)達到50%氣(qì)體體(tǐ)積(jī)(t50)和(hé)66%氣體體積(t66)所(suǒ)需(xū)的混合時間(jiān)。此外,測定(dìng)達到(dào)的(dí)最(zuì)大體積(jī)(Omax)。記(jì)錄10分鐘(zhōng)測(cè)量泡(pào)沫(mò)的塌(tā)陷(xiàn)率,而每1分鐘(zhōng)拍攝(shè)照片。根(gēn)據這些數(shù)據(jù),可(kě)以(yǐ)估算泡沫(mò)的V /。 V /值描(miáo)述當液(yè)體體積達到(dào)其(qí)初(chū)始高(gāo)度的(dí)一(yī)半(排水量)時停止(zhǐ)混合後經過(guò)的時間。
Mechanical mixer (Dispermat) was used to mix hydrophobin, SDS and fibers. Mixer speed was adjustable, but generally about 3800 rpm used in the experiments. Foam volume was studied as a function of time. Foaming reached the maximum level within 1 min with this set-up. The change of foam volume was monitored by photography,where pictures were taken every 10 s. From the photographic images, the mixing times required for reaching 50% gas volume (t50) and 66% gas volume (t66) were determined. In addition, the maximal volume achieved (Omax) was determined.Collapse rate of foam was measured with 10 min recording, whereas pictures were taken every 1 min. From these data V/ for the foam can be estimated. The V/ value describes the time elapsed after cessation of mixing when liquid volume reaches half of its initial height (drainage volume).